Product No.：

LT0110

Synonyms:

Enteropeptidase

Description:

Recombinant EK protease is a common tool enzyme used to excise affinity tags on fusion proteins. It has strong site specificity and strictly recognizes the five-amino acid sequence DDDDK↓. The cleavage site is between aspartic acid and lysine. between. The commonly used five amino acid sequence is: Asp-Asp-Asp-Lys↓.

The recombinant EK protease can achieve the best activity at pH 7.4 and 23 °C, but it is active in a wide range of pH 4.5 to 9.5 and temperature of 4 to 45 °C, so that the choice of reaction conditions can be based on the target protein. Be flexible. In addition, the His-tag contained in rEK can be used to remove it by Ni-NTA resin to achieve the purpose of purifying unlabeled protein.

Sequence:

No data

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Bioactivity:

In 1×rEK Buffer (250mM Tris-HCl, 50mM NaCl, pH7.4, 2.5mM CaCl2), the amount of enzyme required for 50μg of substrate with shearing efficiency>90% was defined as one activity unit after reaction at 23℃ for 16h.