Catalog Number：

LT0077

Synonyms：

Fibroblast growth factor 21

Description：

Fibroblast growth factor 21 is a hormonal protein that in humans is encoded by the FGF21 gene. This protein is a member of the fibroblast growth factor (FGF) family, and specifically a member of the endocrine subfamily which includes FGF23 and FGF15/19. FGF21 is the primary endogenous agonist of the FGF21 receptor, which is composed of the co-receptors FGF receptor 1 and β-Klotho.[5] Loss of β-Klotho abolishes all effects of FGF21.[6]

FGF21 is a hepatokine – i.e., a hormone secreted by the liver – that, apart from regulating various aspects of glucose, lipid, and energy homeostasis, also regulates simple sugar intake and preferences for sweet foods via signaling through FGF21 receptors in the paraventricular nucleus of the hypothalamus and correlates with reduced dopamine neurotransmission within the nucleus accumbens.[7][8][9]

Sequence：

AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Source:

E.coli

Purity:

Greater than 95% by SDS-PAGE gel and HPLC analyses.

Bioactivity:

Assay #1: The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁶units/mg. 
Assay #2: The ED₅₀ was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷units/mg.