Catalog Number：

LT0106

Synonyms:

IFNG, IFG, IFI, nterferon gamma, IMD69

Description:

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells.  IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins.  This promotes the presentation of antigen to T-helper (CD4+) cells.  IFN-γ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response.  Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils.  Human IFN-γ is species-specific and is biologically active only in human and primate cells.  Recombinant Human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues.

Sequence:

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Source:

E.coli

Purity:

Greater than 98% by SDS-PAGE gel and HPLC analyses.

生物活性:

Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED₅₀ for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED₅₀ determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.